Fig. 4. Inhibition of PI-3-K or AKT sensitizes Jurkat leukemia cells to mitochondria-targeted Kv1.3 inhibitors. Jurkat cells were left untreated or treated with 0.25 mM PCARBTP in the presence or absence of the indicated concentrations of the PI-3-K inhibitor LY 294002 or an AKT inhibitor. The cells were incubated for 24 hrs, washed, stained with FITC-Annexin V and cell death was determined by flow cytometry analysis. Given are the mean ± SD of cell death in percent from total cells per sample; n=4, *p<0.05, **p<0.01, ***p<0.001, ANOVA.